20X SSC is a standard molecular biology solution in spiteaft

20X SSC is a standard molecular biology solution; in spiteaftbefst.hat its name implies it\'s 20 times as concentrated as you use it, it actually gets used at a variety of dilutions. For instance, when we wash off the unhybridized probe from the Southern blots, well use 5X SSC. How much 20x SSC and how much water should be mixed in order to make 400 ml of 5x SSC? Hydra express a foot-specific peroxide, so one of the assays I use to determine whether. or. pot an animal has regenerated a foot stain for the activity of this enzyme. My hydrogen peroxide (H2020 stock is 31% (an inconvenient number. I know, but that\'s what it comes as), and I need 0.05% final concentration of H_2O_2 in my staining solution. How much H_2O_2 stock should use to make up 50 ml of staining solution? You have a stock of kanamycin at 100 mg ml and you have made up l liter of medium with which you\'re going to pour plates. If you want the kanamycin concentration in the plates to be 35 mg ml, how much of the kanamycin stock should you add to the flask containing the liter of medium? You also want X-gal in the plates, so you can use a blue white screen for A-galactosidase activity to determine which colonies contain plasmids that are likely to have successfully ligated an insert into the vector, X gal is generally used at 40 HE ml final concentration; if your stock solution is 50 mg ml. how much of it should you add to your 1 liter.

Solution

(1)

Answer:

Stock of SSC= 20x

Required stock = 5x

Volume = 400 mL

C1V1= C2V2

(stock)   (required)

V1=C2V2/C1

V1 = (5x X 400 mL )/ 20x

=100 mL

100 mL of stock + 300 mL uL of H2O gives 400mL of 5x SSC solution