Homework SourseBelow is a diagram of the DNA product of a PCR amplification
Below is a diagram of the DNA product of a PCR amplification of the region of the human genome containing p53. The white region represents the coding region of the gene. The letters above the DNA represent the locations of specific restriction enzyme cut sites. The numbers below each restriction site represent the number of kilogausses (kb) from the first restriction site (at 0 kb) Each of the restriction enzymes creates unique sticky ends. The plasmid into which you want to clone the p53 gene is shown below. Below is a diagram of the PCR product ligated into the plasmid. Drag the restriction enzyme names to the appropriate places on the plasmid diagram. Note that the numbering on this map is different than the original map- the numbering is re-worked after the GOI is inserted into the plasmid. 
Solution
16 kb- B, Since , it was initially present in the Ampr region, it has just been amplified.
19 kb- X, There has been no modification in this region, sheer amplication of the gene X.
4 kb - E, as present in the coding region of the gene. This will not be altered.
9 kb - H, the last restriction enzyme present at the end of the amplified product.
10 kb- S.
