Which of the following mutations is least likely to result i

Which of the following mutations is least likely to result in reduced protein expression?

A. A mutation creating a new splice acceptor (3\' splice site).
B. A point mutation disrupting a splice donor sequence (5\' splice site).

C. A missense mutation in the middle of the open reading frame.

D. A nonsense mutation in the middle of the open reading frame.
E. A missense mutation in the initiator methionine codon.

Solution

B. A point mutation disrupting a splice donor sequence (5\' splice site)

Point mutation is a mutation which affects only one or few nucleotides in a gene sequence. Thus least likely to result in reduced protein expression.