Calculate the volume of buffer to add to a lactase tablet th

Calculate the volume of buffer to add to a lactase tablet that is 9000 FCC units to make a solution at 1000 units/mL concentration.

Solution

It one tablet is 9000 FCC units. Then to get a concentration of 1000units/mL, grind the tablet with a moter and pestle in 4 mL of chilled buffer(0.01 M PO4 buffer, pH 7.). Then make up the volume of this to 9 mL with the same buffer. This 9 mL solution will have a concentration of 1000 units/mL.