What are two methods to localize a protein in a cell What is

What are two methods to localize a protein in a cell?

What is a reporter? What is a reporter gene? What are two examples of reporter genes?

What is a transcriptional fusion? What is a translational fusion? How are these fusion constructs made?

What is PCR? What is typically found in a PCR reaction? How does temperature affect PCR reactions?

What are two sources of template DNA for PCR? How is cDNA generated?

What do restriction nucleases do?

What is a ligation? What is a transformation?

How are transgenic mice expressing either a translational or transcriptional fusion generated?

Solution

Ques-1: What are two methods to localize a protein in a cell?

Answer: Immunohistochemistry

Fluorescent imaging of GFP expression

Fusion protein tags

The above methods are meticulously used for detection specific protein expression localized in a cell. The antibody labeling via primary and secondary immune labeling is going to be useful to find a specific protein. The labeled protein can be detected by fluorescent imaging

Ques-2: What do restriction nucleases do?

What is a ligation? What is a transformation?

Restriction nucleases are the enzymatic proteins acts a molecular scissors useful to cut specific gene sequences in the plasmid or cell genome during recombinant DNA technology

Ques-3: Ligation is the process of addition gene sequences to the specific gene so that a recombinant gene is produced useful for transformation