An Hfr strain of E coli with the genotype gly aziR trS is ma

An Hfr strain of E. coli with the genotype gly^+ azi^R $tr^S is mated with an F-strain of E coli of genotype gly^- azt^5. Glys refers to the amino acid glycine, azi refers to the antibiotic sodium aside, and Str refers to the antibiotic streptomycin, where S is sensitive and R is resistant. Conjugation occurs and the progeny are screened on a selective medium to detect recombinants If you wanted to select foe the F^- recombinant genotype gly^+ axi^T Str^R, you should use a minimal medium containing: Streptomycin Glycine Glycine and streptomycin Streptomycin and sodium azide Glycine sodium azide, and streptomycin P22 phage was used for generalized transduction by growing it on bacteria that were ala^+, pro^+ and The recipient bacteria, which were alla^-, pro^- and tet^+ were infected and incubated on two media minimal media with alanine, and minimal with proline. Colonies that grew were then genotyped and the results are shown below: What is the gene order and which genes are closet together? Choose the best answer The gene order is pro ala tet; tet is closer to pm than to ala The gene order is pm ala tet; tet is closer to ala than to pro. The gene order is pro tet ala, tet is closer to ala than to pro. The gene order is: ala pro tet, tet is closer to ala than to pm. The gene older is, ala pro tet, but the relative distance between the genes cannot be determined from this data In mice, the genes W and Dare tightly linked that virtually no crossovers occur between them. A dihybrid W d/w D is test crossed to a w d/w d tester. The percentage of progeny that are phenotypically WD will be 0 25 50 75 100

Solution

Question 16

the genes W and D are so tightly linked that virtually no crossovers occur between them. A dihybrid Wd/wD is testcrossed to wwdd. The percentage of progeny with W-D- phenotype will be

           Wd/Wd x wwdd

wd

WD

wd WD-     answer

wD

wd wD

Wd

wd Wd

wd/

wd wd/

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15 answer =c

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An Hfr strain of E. coli with the genotype gly+ aziR StrS is mated with an F- strain of E. coli of genotype gly- aziSStrR. Gly refers to the amino acid glycine, azi refers to sodium azide, and Str refers to the antibiotic streptomycin, where S is sensitive and R is resistant. Conjugation occurs and the progeny are screened on a selective medium to detect recombinants. If you wanted to select for the F- recombinant genotype gly+aziR StrR, you should use a minimal medium containing

                A)    streptomycin.

                B)    sodium azide.

                C)    glycine.

                D)    glycine and streptomycin.

                E)     streptomycin and sodium azide. --------answer

                F)     Glycine, sodium azide, and streptomycin.

	wd
WD	wd WD-     answer
wD	wd wD
Wd	wd Wd
wd/	wd wd/