Would would moving the DNA that encodes for an ER targeting

Would would moving the DNA that encodes for an ER targeting signal sequence to the end of the coding sequences from the beginning (N-terminal end of protein) prevent ER targeting?

Solution

No. it will not prevent ER targeting

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Explanation :

signal-recognition particle (SRP) :

it is transiently binds simultaneously to the

ER signal sequence,

And

its receptor on the ER membrane.

Proteins bound for emission have a hydrophobic grouping of amino acids close to their amino-terminal end. A mRNA encoding a protein bound to be discharged ties a free ribosome in the cytosol; Since proteins are incorporated from the amino terminal end towards the carboxy terminal end, the amino terminal end of the proteins develops first from a passage that goes through the huge subunit of the ribosome. A ribosome-related flag acknowledgment molecule examines the incipient polypeptide chain as it rises up out of the ribosome; on the off chance that it experiences a hydrophobic N-terminal singal grouping, it ties it, and stops development of the ribosome along the mRNA.

The ribosome:mRNA:SRP complex then ties to a receptor on the surface of the unpleasant endoplasmic, whereupon protein amalgamation resumes, and the proteins is co-translationally expelled through the layer of the ER into the lumen of the ER, where the hydrophobic flag grouping is separated off by the signal peptidase