1 Your lab manager needs you to make a 05 mgmL Bovine Serum

1) Your lab manager needs you to make a 0.5 mg/mL Bovine Serum Albumin (BSA) in water for a Bradford Assay. At your bench, you have a 2 mg/mL BSA stock. Make 800 L of the desired solution.

2) You collected 900 L of 2 mg/mL BSA in water after a diafiltration step. Now you need to use this solution to make 200 L of protein B at 1.25 mg/mL in 1X buffer. Using the 2 mg/mL BSA stock and the 10X buffer, make the desired solution.

3) To perform a cell count with the hemacytometer, you need at least 20 L of solution, typically 10 L of cell suspension and 10 L of Trypan Blue. This is referred to as a 1:2 dilution. However, when the cell density is too high to count efficiently, the solution can be further diluted with PBS. Adding to the 20 L of cell count solution at your bench, make a 1:3 and 1:10 dilution with PBS.

Solution

1).

Consider the formula, C1V1 = C2V2.

Where, C1 = Concentration of the stock = 2 mg/mL

V1 = Volume of stock to be taken = ?

C2 = Concentration of final volume = 0.5 mg/mL

V2 = Volume of final solution = 800µL = 0.8 mL

Now, substitute the values in the given formula.

2*X = 0.5*0.8

X = 0.2 µL

Take 0.2 µL from the stock solution and makeup the solution up to 0.8 µL with the diluent.