1 You mate two haploid strains of the following genotypes St

1. You mate two haploid strains of the following genotypes:

Strain 1: ADE2 ura3                Strain 2: ade2 URA3

Following sporulation of the diploid, select the spore phenotypes that demonstrate recombination.

If you are having trouble with this question, try some practice problems in Linkage analysis.

2) You sporulate a diploid yeast strain and phenotype the spore colonies. You collect the following data:

16 Thr+ Met+ Trp-

54 Thr+ Met+ Trp+

53 Thr- Met- Trp-

14 Thr- Met- Trp+

Choose the likely genotypes of the two parent haploid strains used in this mating.

If you are having trouble with this question, try some practice problems in Linkage analysis.

a) HOM3 TRP2

c) HOM3 trp2

d) TRP2

e) hom3 trp2

Solution

1.Sporulation of the diploid, select the spore phenotypes that demonstrate recombination is.. Ade-Ura+

2.Genotypes of the two parent haploid strains used in this mating. are..HOM3trp2