11 Eight mutant bacteriophage strains cannot lyse a certain

11. Eight mutant bacteriophage strains cannot lyse a certain type of bacteria that can be lysed by wild-type bacteriophages. The mutant strains were allowed to infect the bacteria in a complementation test. A \"+\" indicates that lysis occurred with coinfection. A \"-\" indicates that lysis did not occur.

(a) A cistron is defined by no complementation in the [Cis or Trans] Configuration.

(b) How many genes are mutated in this experiment? (Use a number not a word in the space) _______________

(c) Which of the following strains below are defective in the same gene as strain 7? Answer yes if the strain is defective or no if it is not.

	1	2	3	4	5	6	7	8
1	-	+	+	+	+	+	-	+
2		-	+	-	+	-	+	+
3			-	+	_	+	+	+
4				-	+	-	+	+
5					-	+	+	+
6						-	+	+
7							-	+
8								-

Answer:

a) Five strains are no complementation with either cis or trans configuration. From the table the follwing strains had no complementation 1&7, 2 &4, 2 & 6, 3 & 5, 4 & 6

b) 23 genes have mutated in this experiment which is shown by the lysis.

c) 1 & 7 are defective and others are not defective with respect to gene 7

11. Eight mutant bacteriophage strains cannot lyse a certain type of bacteria that can be lysed by wild-type bacteriophages. The mutant strains were allowed to

11 Eight mutant bacteriophage strains cannot lyse a certain

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