Defining Genes by Complementation Tests All of the following

Defining Genes by Complementation Tests All of the following are required in complementation testing in yeast except ___. An auxotrophic organism two or more mutations that affect a single phenotype mutations that are recessive diploid cells Two yeast strains are identified. As the result of different mutations, each in a separate gene, each strain is auxotrophic for compound Y, a compound required for growth. Which of the following results would be expected in a complementation test involving these two strains? The cells would not grow if supplemented with compound Y. cells would grow without supplementing compound Y. One strain would require provision of supplement Y, the other would not. Cells would grow only in the absence of compound Y.

Solution

Part A:

All of the following are required in complementation testing in yeast except

Answer is option a. An Auxotrophic organism

Explaination:

Complementation test: Complementation occurs in an organism of 2 different strains with different homozygous recessive mutations that produces the same mutant but wildtype offspring, when mated.

So complementation testing in yeast requires mutations that are diploid cells (we will determine whether the resulting diploid cell requires histidine for its growth or not), recessive, and two or more mutations that affect a single phenotype. Thus Option A is our answer.

Part B:

Two yeast strains are identified. As the result of different mutations, each in a separate gene, each strain is auxotrophic for compound Y, a compound required for growth. Which of the following results would be expected in a complementation test involving these two strains?

Option b. Cells would grow without supplementing compound Y is the result expected in this complementation test involving these two strains