You isolate a new mutation called estuncg that causes rapid

You isolate a new mutation called est-uncg that causes rapid shortening of telomeres in yeast.

In a diploid that is est-uncg/ deletion(est-uncg), the telomere shortening phenotype in even more rapid than in est-uncg/est-uncg homozygotes. What does this result mean?

Would you expect the est-uncg mutation to affect the stability of a circular CEN plasmid in yeast? Why or why not?

Solution

In a diploid strain suppose if one copy of an EST gene is deleted, the amount of Est protein produced will be decreased than it is needed for the wild-type telomere function.

Yes, est-uncg mutation affects the stability of the circular CEN plasmid in yeast. Circular recombinant DNA plasmids contain autonomously replicating sequences (ARSs) present in the extrachromosomal form in transformed yeast cells but these plasmids are highly unstable but the stability can be acheived by the presence of yeast centromere DNA (CEN).