Homework Sourse1 the total space surrounding the gel particle in the packed
1) the total space surrounding the gel particle in the packed column vo=ve of blue dextran why?
2)
calculate the total volume occupied by the column vt using the following equation and data provided
vt=3.14 r2h
vt=?
( inner radius of the column, r= 0.35cm measure the height of the column bed, h=? )
3) inclusions volume of the beads via can be calculated using above values and following equation vt=vi+vo
calculate the I of the beads we used?
Solution
1. Void volume (Vo) = Volume between the gel beads. Most columns can be caliberated for the void volume with a dye, dextran blue, which has an average molecular mass of 2,000,000 daltons.
Elution Volume (VE) = Volume required to elute a particular molecule.
V0= VE in blue dextran so that the molecules to be spearated should easily pass through the gel and does not get disrupted by the dye. Also, the molecular weight of the molecules to be spearated is high, so it allows them to travel easily in the gel.
2. The given equation is: vt= 3.14r2h
Where, r= 0.35 cm
h= 7 cm (Suppose) [ Note: Height is not mentioned, also there is no column]
So, vt= 3.14 X 0.35 X 2 X 7
= 15.368 cm2
