Write the lab procedure for transfection of pglo plasmid in

Write the lab procedure for transfection of pglo plasmid in wukaryotic cells. HOW can i see pglo expression

Solution

We can check the expression of the pglo gene in the eukaryotes cell by quantifying the amount of protein fromed by the gene in the cell by sds page and western blotting to chck for the desired protein..

We can also quantify the amount mRna produced by the gene qTpcr in the cell..

Moreover the pglo gene contains the GFP gene so it can be also be quantified by the amount of flouresnce produced which could be measured by the FRET and FRAP...