1 If you plated 40000 cells on a T25 to make a happy well Ho

1) If you plated 40,000 cells on a T25 to make a \'\'happy\'\' well. How many cells do you need to plate in a 6 well plate? How many cells do you need to plate in a 24 well plate?

2) If you plated 950 cells in a 24 well plate, how many cells do you need to plate in a T25?

3) If you plated 3500 cells on a 6 well plate, how many cells do you need to plate in a 24 well plate? How many cells do you need to plate in a T25?

4) If you plated 50,000 cells in a T25 flask, how many mls of media do you need to add to the flask?

Solution

1. T25 flask contains 5mL medium.

Number of cells = 40000

Number of cells required to load in 6 well plate = 40000 / 6 x 5 = 40000 / 30 = 1333 cells

Number of cells required to load in 24 well plate = 40000 / 24 x 5 = 40000 / 120 = 333 cells

2.

T25 flask contains 5mL medium.

Number of cells = 950

Number of cells required to load in 24 well plate = 950 / 24 x 5 = 950 / 120 = 7.9 cells.

3)

T25 flask contains 5mL medium.

Number of cells = 3500

Number of cells required to load in 6 well plate = 3500 / 6 x 5 = 3500 / 30 = 117 cells.

4)

T25 flask contains 5mL medium.

Number of cells = 50000

Number of ml of media required = 50000 / 5 (1000) = 50000 / 5000 = 10ml