Has no upper limit on size What technique would you use to s

Has no upper limit on size What technique would you use to see if a par lunar sequence is A) on a fragment of DNA? B) present in a sample where it may be at a very low concentration? Name two techniques that can be used to study RNA concentration. You are trying to locate a gene on a plasmid. To do so, you cut it with restriction enzymes, and then run a gel electrophoresis and a Southern Blot. Draw the plasmid map, indicating where your gene of interest is, where each fragment is (Show the length of each one) and where each cut site is given the following information:

Solution

a) A nucleic acid probe can be used to see if a particular seqwuencce is on a fragment of DNA. A probe is a radioactively labelled DNA of variable length that detects the presence of nucleotide sequences that are complementary to the sequence in the probe.
b) DNA hybridization with radioactive probe can be used to detect a sequence present in a sample where it may be in a very low concentration. These probes have complementary nucleotide sequence and are detected using autoradiography.