Which of the following preparations is essentially a pure pr
Which of the following preparations is essentially a pure protein?
Proteins present in the serum-free culture medium of a cell
Cytoplasmic soluble proteins
Proteins secreted by a cell
Nuclear proteins
None of these
Solution
Cytoplasmic soluble proteins
To purify a pure protein fractionate the cell into components and determine which component is enriched in the protein of interest. the fractionation yielding a dense pellet of heavy material at the bottom of the centrifuge tube and a lighter supernatant above. the supernatent is cytoplasmic soluble proteins that can be paas through a affinity purification column and we can get a pure protein.
