Say you have an organism with a genome of 1 thousand bases 1
Say you have an organism with a genome of 1 thousand bases (103) and a forward primer made up of 4 nucleotides (aka, a 4-mer).
Please write out your work, but no need to do the arithmetic until part (f).
A. How many possible 4-mers are there? Remember that each position in the 4-mer has one of four nucleotides.
Solution
A. Since each of the 4 bases occupy one location at a given time, the number of possible combinations will be 4! i.e. 24

