Say you have an organism with a genome of 1 thousand bases 1

Say you have an organism with a genome of 1 thousand bases (103) and a forward primer made up of 4 nucleotides (aka, a 4-mer).

Please write out your work, but no need to do the arithmetic until part (f).

A. How many possible 4-mers are there? Remember that each position in the 4-mer has one of four nucleotides.

Solution

A. Since each of the 4 bases occupy one location at a given time, the number of possible combinations will be 4! i.e. 24

Say you have an organism with a genome of 1 thousand bases (103) and a forward primer made up of 4 nucleotides (aka, a 4-mer). Please write out your work, but n

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