Which is the last to happen during DNA replication Which is

Solution

The last step of replication is \"termination\":

DNA polymerase II is an enzyme that essentially enables DNA replication in semiconservative manner & during termination, polymerase reaches the end of the strands as described below

Nucleases are going to perform proof reading to remove the wrong nucleotides followed by filling of gaps by DNA polymerase

Mechanism:

DNA template (lagging strand) read by primase, which synthesize complementary RNA primer. DNA polymerase extends the primed segments forming the Okazaki fragments, now the RNA primers removed. The synthesis of lagging stand occurs using short DNA segments called Okazaki fragments each consisting of 100 to 200 nucleotides in humans (1000 to 2000 nucleotides in bacteria). The replication occurs in 5 to 3 direction, but away from the replication fork. DNA ligase connects the Okazaki fragments by catalyzing the formation of phosphodiester bond between the Okazaki fragments of complementary strand.

DNA polymerase I: Proof reading

Polymerase would replace longer stretches of DNA.

Association of beta clamp with pol I would affect increase the activity of DNA polymerase I, which can replace longer stretches of DNA.