Based on the figures below which of the expressions would be

Based on the figures below, which of the expressions would be correct?

A) V_max = 1/B

B) C = 1/ V_max

C) D= V_max

D) D = 1/ V_max

E) A = 1/ V_max

Solution

Answer: (D) D = 1/Vmax

A plot of 1/V0 versus 1/[S] is called the Line-Weaver Burk plot or Double reciprocal plot. It yields a straight line with an intercept of 1/Vmax and slope of 1/Vmax. The intercept on the X-axis is -1/Km.

Vmax can be accurately calculated if the Michaelis-Menten equation is transformed into one that gives a stright line plot. Thus,

1/V0 = 1/Vmax + (Km/Vmax) * 1/[S]