You have isolated five different homozygous recessive mutant

You have isolated five different homozygous recessive mutant lines that all produce the same phenotype, and now wish to determine if the mutations affect the same gene. To do this, you cross the mutants together in a complementation test. A \"+\" means that there was complementation, and a \"-\" means you observed the mutant phenotype. a. Are w3 and w4 alleles of the same gene? b. How many different \"complementation groups\" (i.e. genes) have you isolated? c. Sort the mutants into different complementation groups.

Solution

a. No. W3 and W4 are allels of different gene

b. 9 different complementation groups

c. W1W1,W1W3, W2W2, W2W4,W2W5,W3W3,W4W4,W4W5,W5W5