Consider the following questions in your writeup o Suppose y

Consider the following questions in your write-up: o Suppose you are viewing a Gram stained field of red rods and purple cocc through the microscope. What can you conclude? o Why must the spore stain include a hearing step? What would happen ifthis was omitted? o Why is staining bacterial components useful in strain identification?

Solution

Answer 1. Rods will be gram negative and cocci are gram positive .Slides were made from culture of bacteria or culture was contaminated.Purple hold the stain due to its unique membrane it contain peptidoglycan layer that act as lattice trapping crystal violet iodine dye.

Answer 2: Heat step is necessary while staining spore because application of heat disrupt vegetative cells.

Answer 3. Staining can help in distinguishing various kind of bacteria.Gram positive bacteria will take crystal violet stain due to its membrane properties. While those which never take such stain are gram negative bacteria they take counter stain sefranin they are stained pink.