You set up the following gel in an attempt to construct a re

You set up the following gel in an attempt to construct a restriction map of the plasmid pUK1, using the restriction endonucleases BanII and Bg/II. This series of questions is concerned with the principles of electrophoresis underlying this study. All digests were performed on circular DNA, which if uncut give no sixe information as they are a mixture of plasmids coiled by different amounts... What is the number of restriction sites of Banll in pUK1? 1 2 3 4 5

Solution

12.

(B) 2

Since we see 2 bands in lan 3, it could be that BanII has two restriction sites on pUK1. Two cuts in a ciircular plasmid result in two fragments hence two bands