For the following problems you need to know the ratio of the

For the following problems, you need to know the ratio of the diluent to the sample aliquot. For instance, if you are making a 1:20 dilution, the ratio of diluent to aliquot will be 1 unit less than the dilution factor, i.e., 20 parts total, 19 parts diluent, 1 part sample aliquot. 7) You have 0.6 mL of sample, and want to dilute it all to a fiftieth (1:50) of its current concentration. How much diluent will you add? What will the final volume be?

Solution

For making a dilution of 1:50 for the sample of 0.6 mL, we should have 1 part sample and 49 parts diluent.

So, there should be 0.6 mL sample and 0.6*49 = 29.4 mL diluent.

Total final volume is 0.6mL and 29.4 mL = 30.0 mL.