the unknown protein con (14 points) This following experiment investigates the determines the effect of varying th e enzyme concentration. activity of an enzyme peroxidase and of hydrogen peroxide he production of water Peroxidase catalyzes the breakdown colorless to a colore pon oxidation, a property and oxygen Certain dyes will go from a u activity. In the presence of hydrogen peroxide and peroxidase, on that allows detection of peroxidase hophenylenedi amine (OPD) will be changed from a colorless dye to a yellow compound as it is oxidized. peroxidase 11,02 + OPD-112 H2O + OFFD (colorless) (yellow) (reduced fonn) (oxidized form) Using a spectrophotometer, you quantitated the enzyme reaction by measuring the absorbency time period, the from the oxidized OPD at 4 1 Ornn. The more colored OPD produced in a giv en test tubes (Table 1) faster faster the e nzymatic reac and the absorbance readings.
A. Peroxidase Enzyme act on hydrogen peroxide and converts it into water (H2O) and oxygen (O2) and this oxygen change colourless dye into yellow colour,As amount of enzyme increases more reaction take place in reaction and more absorption observe.
B.Hydrogen peroxide (H2O2).
Homework Sourse
