n 20 melting temperature of a population of DNA molec percen

n 20 melting temperature of a population of DNA molec percen the DNA becomes single-stranded. Consider the defined oligomers: Double Stranded Melting Temperature c Length lin Oligomer 37.9 CAAAAAAAG 40.3 a. Complete (fill in) the table above. hydrogenbonde b. Do the melting correlate with the number of bones, the temperatures more H- n pera ture son Higher order Structure of Nucleic Acids structural use DNA cal ling uite likely erhel local and chang ons, following

Solution

Table :1

a) complete the table below:

Number of b.p :

1. 9 bp

2. 10 bp

3.   9 bp

b. yes, double strand DNA helix complementary strand due to A-T and G-C pairing. Hydrogen bond can occur simultaneously at two site within adenine/thymine pairs. Likewsie three hydrogen bonds can form in G-C pairs, we need more energy to break GC content compared to AT bp .   It means that high GC content indicate high melting temperature of DNA.

Table :2

d) compelte given table below:

Number of Hydrogen bonds: [ A = T ; G =_ C ]

Given sequences : CAAAGAAAG i.e, (3+2+2+2+3+2+2+2+3= 21 Hydrogen bonds

e) In all sample is same is length of bp , different is number of hydrogen bonds and Melting temperature of DNA

f) Observation:

1.Top two rows having similar bp in length, and number of hydrogen bonds but different Melting temperature.   High GC of oligmer having high Tm

2. Bottom two rows having similar in bp in length, Tm but number of hydrogen bonds are different.