Your labmate plans to create a transcriptional fusion of the

Your labmate plans to create a transcriptional fusion of the gene hetN to GFP. They want to use GFP as a transcriptional reporter to determine when hetN is transcribed during normal growth of Anabaena. However, they are insistent that GFP has to be in the same ORF as hetN in order for it to be transcribed. Are they correct? Why or why not? What about if they wanted to make a translational fusion, does GFP and hetN have to be in the same ORF? Why or why not?

Solution

Explanation :-Yes they are cirrect for any transfusion protein to be functional they must be in the same open reading frame. Here GReen Flourscent Protein and hetN must transcribed simultaneously in the same open reading frame in the Anabaena. The result would be the fusion protein having all the amino acids translated fused woth GFP that would determine wheb the target protein would be transribed in the cell.