Predict the effect of the NADPHNADP ratio as compared to con

Predict the effect of the NADPH/NADP+ ratio (as compared to controls) in cells under the following circumstances:

a. Withdrawal of serum.

b. Addition of DHEA.

c. Addition of H2O2.

d. Withdrawal of serum and addition of H2O2

Solution

a. Withdrawal of serum : NADPH/NADP+ ratio decreases as glucose-6-phosphate dehydrogenase (G6PD) enzyme activity decreases with withdrawal of serum.

b. Addition of DHEA :There is a decrease in NADPH/NADP+ ratio as DHEA inhibits glucose-6-phosphate dehydrogenase (G6PD).

c. Addition of H2O2.NADPH/NADP+ ratio increases as addition of H2O2 enhances the activity of pentose phosphate pathway thereby increasing the NADPH/NADP ratio.

d. Withdrawal of serum and addition of H2O2: Not much change in NADPH/NADP+ ratio.