What is the difference between Western BlotImmunoblot vs Imm
What is the difference between Western Blot/Immunoblot vs Immunoprecipitation and what would be a scenario to use each one? From my understanding, western blot/immunoblot used for protein expression and Immunoprecipitation is used to look at protein interactions is that correct?
Solution
Yes, you are right.
The primary use of immunoprecipitation is to demonstrate a physical association between two proteins.
Western blot is used to identify specific proteins from a complex mixture of proteins and quantification of the same in relative to constitutively expressed protein.
