To test the effects of this protease you perform an experime

To test the effects of this protease, you perform an experiment by including the protease with protein B, C and C, and you run a page gel (show below) to visualize the results. Lane MW shows molecular weight standards. Lane A shows a negative control. What is one example of a sample can you run in lane A as a negative control? What is the purpose of the negative control?

Solution

The purpose of usage of negative control in PAGE is to know the absence of target cell/tissue in our sample not to know the expression of required target cell in our sample and this step is useful in know/ check the non specific binding sites and tofind false positive data/results.

Example for running negative control - PD1 proteins which are absent in various organs such as placenta, kidney, heart etc., COS cells having vector, fr-1-V5 transfected HEK293T cells etc can be used.