why is sds omitted when proteins need to undergo isoelectric

why is sds omitted when proteins need to undergo isoelectric focusing

Solution

Answer:

Isoelectric focusing separates proteins according to their Isoelectric points in a pH gradient. Isoelectric point is the pH at which protein carry no net charge, or pH at which protein become immobile in an electric field. SDS is a detergent which when binds to proteins gives them net negative charge. Therefore, affects the native charge on the protein and affects isoelectric focusing of proteins.