Describe the key experiments that supported the semiconserva

Describe the key experiments that supported the semi-conservative model of DNA replication in E. coli.

Solution

DNA REPLICATION:Producing two copies of dna from a single dna molecule is called dna replication...

SEMI CONSERVATIVE MODEL OF DNA REPLICATION IN E.COLI:

1)In this model each strand of dna serves as a template to make a new complementary strand.

2)This model made a lot of sense given the structure of the DNA double helix, in which the two DNA strands are perfectly, predictably complementary to one another (where one has a T, the other has an A; where one has a G, the other has a C; and so forth).... This relationship made it easy to imagine each strand acting as a template for the synthesis of a new partner.

They began by growing E. coli in medium, or nutrient broth, containing a \"heavy\" isotope of nitrogen, N15Nstart superscript, 15, end superscript, N. (An isotope is just a version of an element that differs from other versions by the number of neutrons in its nucleus.) When grown on medium containing heavy text N15Nstart superscript, 15, end superscript, N, the bacteria took up the nitrogen and used it to synthesize new biological molecules, including DNA.

it is most useful method......