The graph below shows how enzymes from two different organis

The graph below shows how enzymes from two different organisms are affected by increasing temperature. The enzyme activity is reported as the rate of the reaction - the higher the rate, the more active and more rapidly the chemical reaction occurs that is catalyzed by the enzymes. The curve defined by the brown volume (left) illustrate the average activity of human enzymes and the curve defined by the red volume (right) illustrates activity of enzymes from a bacterium that lives in hotsprings.

the human

the hotsprings bacteria

folded

denatured

secondary structure

primary structure

None of the above, the fold of the human enzymes is stable at 50 C.

tertiary structure

A) Which organism has enzymes most active at temperatures above 50 C? the human the hotsprings bacteria

B) At 70 C, would you expect the human enzymes to be in a folded or a denatured state? folded denatured

C) Considering the human enzymes at 50 C, which level of protein structure would be most destabilized? secondary structure primary structure None of the above, the fold of the human enzymes is stable at 50 C. tertiary structure

Optimum temperature Optimum temperature for human enzyme for enzyme from hotsprings bacterium 30 40 50 60 70 80 Temperature of reaction (OC)

Solution

A.  Which organism has enzymes most active at temperatures above 50 C?

Hot spring bacteria

It survives in Hot springs and hence the name hotspring bacteria.

B.At 70 C, would you expect the human enzymes to be in a folded or a denatured state?

Denatured.

The bonds are broken and hence the enzyme denatures.

C.Considering the human enzymes at 50 C, which level of protein structure would be most destabilized?

Tertiary structure .