When asked to analyze your lob results be specific ie refer

When asked to analyze your lob results, be specific (i.e. refer to your test results in your answer). Be sure to cite your source(s) used. If a clinical laboratory test is highly specific then it will distinguish between diseases (or pathogens) that may look alike or be closely related. For example, an influenza test may be described as highly specific because it can distinguish between Influenza type A and Influenza type B. Explain why an ELISA test is typically described as \"very specific\". Why is the ELISA test that you simulated known as an \"indirect\" diagnostic test? Distinguish between the terms: antigen and pathogen. Immune System and ELISA Simulation Lab

Solution

1. This method does not require fractionation of the sample and based on the property of proteins to readily to a plastic surface. ELISA is used in both experimental and diagnostic virology. It is a highly sensitive assay that can detect proteins at the picomolar to nanomolar range. ELISA involves at least one antibody with specificity for a particular antigen.

2. Indirect ELISA is a two-step ELISA which involves two binding process of primary antibody and labelled secondary antibody. The estimation of antigen is done based on the amount of secondary antibody bound to the primary antibody which is an indirect way. The technique has high sensitivity compared to other ELISA.

3. Antigen: It is a molecule which can evoke an immune response in a body

Pathogen: It is a molecule or organism which can cause disease in a body.