What can be concluded from these specific activities when co

What can be concluded from these specific activities when comparing the wild-type with this mutant?

Glutathione transferases are enzymes that catalyze the reaction of glutathione with electrophiles of both endogenous and xenobiotic origins. The main biological roles of GSTs encompass detoxification and protection against oxidative stress. By conjugating glutathione with toxic electrophilic substrates, the resulting molecules generally become less reactive and more soluble, thus facilitating their excretion from cells and the organism. Site-directed mutagenesis was used to study the role of Arg111 in substrate binding and catalytic activity, specific activities of wild-type and Arg111A1a mutant are given below. What can be concluded from these specific activities when comparing the wild-type with this mutant?

Solution

Answer:

Glutathione S-transferases (GSTs), comprise a family of enzymes best known for their ability to catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrate for the purpose of detoxification.

GST act as modulators of signal transduction pathways that control cell proliferation and cell death.

Release of GSTs as an indication of organ damage.

The high intracellular concentrations of GSTs coupled with their cell-specific cellular distribution allows them to function as biomarkers for localising and monitoring injury to defined cell types.

Any tissue damage, injury or viral infection or ROS (reactive oxygen species) production can be assayed by using wild type verses mutant GST.

Cell proliferation and cell death assay can be done using both wild and mutant type to check the wild type vs mutant GST as GST involved in the inhibition pathway of MAPK analysis.