4 Calculate the following if initial bath volume in 20 mL Ad
4. Calculate the following if initial bath volume in 20 mL:
Add 0.2 mL of 10^6M ACh to bath yields [ACh] in the bath:
Add 0.2 mL of 10^5M ACh to bath yields [ACh] in the bath:
Add 0.2 mL of 10^4M ACh to bath yields [ACh] in the bath:
Solution
4. Calculate the following if initial bath volume in 20 mL:
Add 0.2 mL of 10^6M ACh to bath yields [ACh] in the bath:
Fold dilution = (Stock volume + diluent volume)/ stock volume
= (0.2 + 20 )/0.2
=101
Final Concentration of ACh = Stock conc./fold dilution
= 10^6M/101
= 0.99 x 10^4M
Add 0.2 mL of 10^5M ACh to bath yields [ACh] in the bath:
Final Concentration of ACh = Stock conc./fold dilution
= 10^5M/101
= 0.99 x 10^3M
Add 0.2 mL of 10^4M ACh to bath yields [ACh] in the bath:
Final Concentration of ACh = Stock conc./fold dilution
= 10^4M/101
= 0.99 x 10^2M
![4. Calculate the following if initial bath volume in 20 mL: Add 0.2 mL of 10^6M ACh to bath yields [ACh] in the bath: Add 0.2 mL of 10^5M ACh to bath yields [A 4. Calculate the following if initial bath volume in 20 mL: Add 0.2 mL of 10^6M ACh to bath yields [ACh] in the bath: Add 0.2 mL of 10^5M ACh to bath yields [A](/WebImages/12/4-calculate-the-following-if-initial-bath-volume-in-20-ml-ad-1012480-1761522791-0.webp)