4 Calculate the following if initial bath volume in 20 mL Ad

4. Calculate the following if initial bath volume in 20 mL:

Add 0.2 mL of 10^6M ACh to bath yields [ACh] in the bath:

Add 0.2 mL of 10­^5M ACh to bath yields [ACh] in the bath:

Add 0.2 mL of 10­^4M ACh to bath yields [ACh] in the bath:

Solution


4. Calculate the following if initial bath volume in 20 mL:

Add 0.2 mL of 10^6M ACh to bath yields [ACh] in the bath:

Fold dilution = (Stock volume + diluent volume)/ stock volume

= (0.2 + 20 )/0.2

=101

Final Concentration of ACh = Stock conc./fold dilution

= 10^6M/101

= 0.99 x 10^4M


Add 0.2 mL of 10­^5M ACh to bath yields [ACh] in the bath:

Final Concentration of ACh = Stock conc./fold dilution

= 10^5M/101

= 0.99 x 10^3M

Add 0.2 mL of 10­^4M ACh to bath yields [ACh] in the bath:

Final Concentration of ACh = Stock conc./fold dilution

= 10^4M/101

= 0.99 x 10^2M

4. Calculate the following if initial bath volume in 20 mL: Add 0.2 mL of 10^6M ACh to bath yields [ACh] in the bath: Add 0.2 mL of 10­^5M ACh to bath yields [A

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