Propose a rational study design for developing a novel image

Propose a rational study design for developing a novel image analysis software with the ultimate goal to detect two cancerous regions of diameter <0.5 μm and located 0.5 μm apart in a thin tissue sample of liver. Assume that the thin tissue is stained with a fluorescence contrast agent that emits light at 543 nm, and the tissue is mounted on aslide. Discuss microscopy imaging, image analysis protocol, and a method for performance evaluation of the developed software  

Solution

microscopy imaging

microscopy imaging technique which benefits from differences in the light refraction by different sections of living cells and transparent specimens and allows for better visibility during microscopic evaluation.

first developed in 1893 through the optimization of a microscope’s optical train so as to allow for homogenously bright light without artifacts and glare.

his imaging technique also uses stains so as to better view more components and details of the inner structures of cells

image analysis protocol

image analysis protocol that identifies regions of interest (ROIs) using a set of empirically determined rules. The relative expression levels of both fluorescent proteins are estimated, and YFP-NCoR subnuclear organization is quantified based on the mean focal body size and relative intensity. The selected ROIs are tagged with an identifier and annotated with the acquired data. This integrated image analysis protocol is an unbiased method for the precise and consistent measurement of thousands of ROIs from hundreds of individual cells in the population.

method for performance evaluation

Performance evaluation, or performance appraisal (PA), is the process of assessing an employee\'s job performance and productivity. The assessment is conducted based on previously established criteria that align with the goals of the organization.

Various employee attributes can be assessed during this process, including organizational-citizenship behavior, accomplishments, strengths and weaknesses, and potential for future improvement. The management of performance plays a vital role in the success or failure of the organization, as human resources are a significant investment that must provide meaningful returns. An ineffective performance-evaluation system can create high turnover and reduce employee productivity.