molecular biology help these are only 1 to like 3 word answe

molecular biology help

these are only 1 to like 3 word answers, i want to make sure my answers are right, thanks soo much

1. In Frances Crick\'s hypothesis about the central dogma, what did he propose to be the intermediate carrier? 2. In Brenner and Jacob\'s experiment, old ribosomes were made heavy with which two isotopes? 3. Which form of prokaryotic RNA polymerase has a sigma subunit? 4. Which subunits of prokaryotic RNA polymerase bind to the UP element? 5. Fill-in-the-blank. The core enzyme of RNA polymerase by itself can transcribe DNA only if the DNA is 6. When the core enzyme of RNA polymerase by itself transcribes DNA (under the conditions of question #5), it will transcribe (only one or both, choose one answer) DNA strand (s) 7. In Hinkle and Chamberlin\'s experiment, which method did they use to measure binding between RNA polymerase and promoter DNA?

Solution

Answer:

1. RNA is an intermediary between DNA and protein

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2. in brenner and Jacobs experiment old ribosomes wer made heavy with two isotopes

a) Heavy isotopes of carbon (¹³C) and nitrogen (¹⁵N).

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3. RNAP : the core of RNAP associates with the transcription initiation factor sigma ()

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4 ^I and ^II SUBUNITS RNAP

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5, core is unable to efficiently bind to a promoter

or

initiate RNA synthesis without a sigma subunits

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6. ONE : template-strand sequence into RNA sequence

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7. labeled DNA (radioactive)

Mixtures were filtered through nitrocellulose .

It allowing to monitor dynamics of binding and dissociation