Bacterial cells need to tightly regulate DNA replication so

Bacterial cells need to tightly regulate DNA replication so that replication does not Initiate multiple times before cell division occurs. The cells use several methods to regulate the initiation of DMA replication. In order for replication to initiate which of the following needs to occur? DnaA Is not bound to Orica and the 6ATC sites 011 one strand are methylated. DnaA is not bound to one and the GATC sites on Doth strands are methylated DnaA Is hound to Orica and the GATC sites on one strand are methylated. DnaA is bound to Orica and the GATC sites on both strands are methylated

Solution

D. DnaA is bound to oric and the GATC sies on both strands are methylated.

DnaA (required for initiation of replication) binds less well to hemimethylated DNA. As a result, newly replicated origins are prevented from immediately initiating another round of DNA replication and they are completely methylated dnaA start the replication.