you have run out of imidazole in the lab How else could you

you have run out of imidazole in the lab. How else could you elute your His-tagged GFP off of a Ni-column?

Solution

The most other way to remove the his-tagged GFP from a Ni-columnn is to change the pH gradient. The change in the pH gradient offers elution to the protonated his-tagged GFP protein from basic to acidic conditions which results in removing the protein from the Ni-columnn..

As the pH changes the affinity of binding to the Ni-columnn also changes due to which the protein gets separated from it.