Homework 4 10 pts Show all work neatly including units Answe

Homework 4 (10 pts) Show all work neatly, including units Answer the following questions based on the bacterial transformation experiment (LAB 4) 1) Explain the difference between the DNA\" and DNA\" conditions of this experiment, as you would for someone who was not familiar with BIO325. (2pts) 2) Also during the transformation experiment, you plated two different amounts, (10uL and 100uL), of each sample under each growth condition. Some hypothetical data from this experiment is presented below. Assume that no technical or procedural errors occurred during the experiment. DNA\" 10uL DNA\" 100uL. LB AMP LB AMP What is the point of plating different volumes of each transformation condition (why did you do this?). (2pt)

Solution

1). The positive sense strand of DNA or the + DNA is the one, whose version is translated into RNA, means the same sequence is translated into RNA, except that Uracil comes in place of thymine. The sense strand runs in 5\' to 3\' direction.

The anti-sense strand or -DNA is the complimentary strand of + DNA, which runs in 3\' to 5\' direction.