molecular biology help please 8 In Travers and Burgess exper

molecular biology help please!

8. In Travers and Burgess\' experiment looking at the function of the sigma subunit with nucleotides labeled with either C-14 or P-32, what was their independent variable (variable on the X-axis of their dose-response curve? 9. When Travers and Burgess used C-14 labeled nucleotides, which process(es) of transcription did they measure? 10. If one is using radioactive nucleotides (e.g., ATP, UTP, GTP or CTP) to label RNA, which atom in the nucleotide is radioactive in order to measure transcription initiation only? Be specific. 11. Which antibiotic binds RNA polymerase to block transcription initiation? Spelling counts

Solution

8.The independent variable is nucleotide labelled with either C-14 or P-32 as this is an input that is given to study the effect i.e function of sigma subunit.

9.They measured the initiation of transcription process as sigma subunit is a protein subunit to which RNA polymerase binds to initiate the transcription process.

10.The phosphate atom in the nucleotide is radioactive in order to measure transcription initiation only.

11.Rifampicin is the antibiotic that binds RNA polymerase and blocks transcription initiation.