Lab instructions You will be given a growing culture of 3T3

Lab instructions: You will be given a growing culture of 3T3 fibroblasts to maintain. Your job will be to keep the culture alive, keep the media free of contamination, monitor cell growth, and sub-culture the cells when needed. Your first task will be to determine the concentration of the cells given to you and seed 6-well plates at 1 x 105 cells/well.

QUESTION: How many cells/ml are present in your culture if you count the following number of cells in 5 different 4x4 areas on a hemocytometer using a 1:10 dilution of your culture? 37, 36, 29, 45, 53.

Solution

Total cells/ml= Total cells counted x (dilution factor/ number of squares) x 10,000 cells

= 200 x (10/5) x 10000

=200 x 2 x 10000

= 4000000

=400x10⁴cells