How much stock and diluent do you mix to make 6 L of solutio

How much stock and diluent do you mix to make 6 L of solution from a 25 X stock? After spring start doing experiments treating cells in culture with cuff cine to study the effects of caffeine. Today, we w.11 prepare concentrated stocks of caffeine to us future experiments. in a group of 4. you will prepare a 10 ml of a 1M Mock of caffeine. What is the molecular weight of caffeine? How many grams of caffeine do you need to have 1 mole of caffeine molecules? Calculate the number of grams of caffeine that you will need to dissolve into a solvent to make 10 mL of a 1 M solution of caffeine: Have an instructor check your math before you move on* We will make our caffeine solutions using water as the solvent. Make your 10 ml of 1 M solution of caffeine using a 15 ml conical tube. you may need to put your tube into the hot water in the beaker on the hot plate to help the caffeine go into solution. How will you know that the caffeine is in solution (what will your solution look like)?

Solution

1.Since the concentration is not mentioned, we will assume it to be 1X.

C1V1= C2V2

25 * V1 = 6X *1L

V1 = 0.24 ml

Take 0.24 ml from stock and add 5.76 diluent.

2.Caffeine Solution:

Molecular weight of caffeine is:194.19 g/mol

To have 1 mole of caffeine molecules we need to have 194.19 g of caffeine.

Molarity = weight * 1000 / molecular weight * Volume in ml

1M = weight * 1000 / 194.19 *10

weight = 1.94 g ( ans )

On addition of water to caffeine followed by heat, caffeine will dissove completely in water and the solution will be homogenous.This will confirm that caffeine is in solution.