On a particular microscope the numerical apertures of the co

On a particular microscope, the numerical apertures of the condenser and medium power objective lenses are 1.25 and 0.25, the high dry lens has a numerical aperture of 0.85. What is the limit of resolution? Will you be able to distinguish two points that are 250 nm apart as being separate, or will they blur into one?

Solution

Limit of resolution(D) = wavelenght/NAc + NAo condenser and medium power objective lenses are 1.25 and 0.25 dry lens has a numerical aperture of 0.85 two points that are 250 nm apart filter with wavelength = 520nm D = 520 / (1.25+ 0.85) = 520/(2.1) = 247.61 nm It is possible to distinguish two points that are 250 nm apart as being separate because the distance between them is more than 247 nm.