Thank you A 13nucleotide long DNA template was sequenced by
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A 13-nucleotide long DNA template was sequenced by the chain-terminator method (the Sanger method) using a 5\'-TAG primer. The resulting fragment analyzed by gel electrophoresis as shown below. In each of the four different gel lanes (or wells), a different ddNTP was used. Determine the sequence of the DNA template strand. Solution
So starting from the lower end, which is the 5\' end,
we have first nucleotide as A, then G, G, C.....
Hence, 5\'- AGGCTCAGCA -3\' is the sequence.
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