if a myoglobin has an OD409 value of 1 what percentage of li

if a myoglobin has an OD409 value of 1, what percentage of light does it transmit at 409 nm? What pecentage of light does it absorb at 409 nm?

Solution

Myoglobin OD409 is 1, this shows that for wavelength 409 nm, OD = 1.

According to Beer Lamberd Law,

Percentage transmisson = 10^-OD x 100 = 10^-1 x 100 = 10 %.

As transmisson is 10%, then absorption percentage = (100 - 10) = 90 %.