Which parameters of the MM equation relates to the affinity

Which parameter/s of the MM equation relates to the affinity of an enzyme for its substrate (mark all that apply)? V_0 K_m V_max Slope of the line A higher V_max must mean a higher K_m. FALSE; There is no relationship between V_max and K_m. TRUE; K_m = 1/2 V_max TRUE; V_max = 1/2K_m FALSE; V_max is not just twice K_m Which of the following are assumptions that we make in order to derive the MM equation (mark all that apply)? [P] = 1 Molar The [S] >>>> [E] The reaction is at steady state The reaction is at equilibrium

Solution

1. V₀ is the parameter that relates the relation ship between enzyme and substrate as it denotes the rate of catalysis or the number of substrate molecules that are converted into products.V max denotes the saturation of the enzyme with the substrate molecules.

2.False higher V max means always lower Km it is alwys half of Vmax.

3. The reactioin is at steady state.