3 ml of assay buffer is expected to generate 03 moles ONP pe

3 ml of assay buffer is expected to generate 0.3 moles ONP per minute. How many moles of ONP will be in the assay tube 2 minutes after -galactosidase is added? Note that the assay buffer is composed of 0.1 M sodium phosphate (pH 7), 2 mM MgSO4, 0.1 M -mercaptoethanol, and 1 g -galactosidase.

a) 0.001 moles ONP

b) 0.15 moles ONP

c) 0.3 moles ONP

d) 0.6 moles ONP

e) 0.42 moles ONP

Solution

d) 0.6 moles ONP

the generation of ONP will increase linearly with the time of assay. So it will relase 0.6 moles of ONP after 2 minutes in the tube.