What observations would allow you to conclusively determine

What observation(s) would allow you to conclusively determine that you are studying an uncompetitive inhibitor (and no other type of inhibitor)?

The inhibitor binds to the enzyme at some location other than the active site.

The inhibitor decreases the maximal velocity of the enzyme.

The inhibitor only interacts with the enzyme when the substrate is bound.

All of the above would allow you to conclusively determine you have an uncompetitive inhibitor.

Solution

All of the above would allow you to conclusively determine you have an uncompetitive inhibitor. It is true that the Inhibitor (I) will only bind to the enzyme (E) once a enzyme substrate (S) complex (ES) is formed, this Enzyme substrate complex brings about conformational changes in the enzyme which facilitates the binding of the inhibitor thus forming enzyme substrate inhibitor complex (ESI) this decreases the maximum velocity and no product will be formed.